Engineering a Circular Riboregulator in Escherichia coli
Diagram of group I and PIE ribozymes [14, 18] and mechanism of riboregulation. (a) Left: secondary structure of wild-type group I intron, with the dispensable P6 loop colored in green, introns in pink, and exons in blue. Right: structure of the PIE ribozyme, with riboregulator RAJ31  inserted between the exons. (b) Sequence scheme in the wild-type group I ribozyme and illustration of products after self-splicing. (c) Sequence scheme in the PIE ribozyme, where the order of the introns and exons is inverted, and illustration of products after self-splicing, including the circularized sRNA. (d) Diagram of riboregulation with a circular sRNA. A cis-repressed mRNA is trans-activated by the circular sRNA, which induces a conformational change in the 5 UTR to release the RBS and then allow translation initiation (see sequences and structures of our design in Figures S1 and S2).
Please note that the Table of Contents page is currently not displaying content. We are working to fix this issue as soon as possible and apologize for the inconvenience.