BioDesign Research / 2020 / Article / Tab 2

Review Article

Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research

Table 2

Comparison of prime editing with other gene editing technologies.

Areas of applicationsPrime editingBase editingCRISPR-Cas9TALENsZFNs

Generation of single point mutation✓ (via HDR)✓ (via HDR)✓ (via HDR)
Simultaneous introduction of multiple point mutations×✓ (via HDR)✓ (via HDR)✓ (via HDR)
Precise insertion×✓ (via HDR)✓ (via HDR)✓ (via HDR)
Precise deletions×✓ (via HDR)✓ (via HDR)✓ (via HDR)
Simultaneous introduction of insertion and deletions×✓ (via HDR)✓ (via HDR)✓ (via HDR)
Substitution (transition type)✓ (via HDR)✓ (via HDR)✓ (via HDR)
Substitution (transversions)×✓ (via HDR)✓ (via HDR)✓ (via HDR)
Directed gene evolution××
Generation of gene knockout
Modification of cis elements×✓ (via HDR)✓ (via HDR)✓ (via HDR)
Gene activationaLimited scaleLimited scaleLimited scaleLimited scale
MultiplexingNot tested yetLimited scaleLimited scale

CRISPR: clustered regulatory interspaced short palindromic repeat; Cas: CRISPR associated; TALENs: transcription activator-like effector nucleases; ZFNS: zinc finger nucleases; HDR: homology directed repair. aGene activation: here, gene activation means the restoration of the activity of a gene that has mutation in the coding sequence. “” indicates “extremely difficult or inefficient,” “✓” indicates “capable,” and “×” indicates “not capable”.