Characterization of silenced cells. (a) Assessment of DNA methylation (A) and H3K9me3 (B) of cells exposed to dCas9-KRAB+dCas9-DNMT3A+dCas9-DNMT3L versus untreated cells near reporter promoter. Binding sites of dCas9 are indicated by red lines. (b) Reactivation of silenced cells using various domains fused to the N-terminus (pink) or C-terminus (blue) of dCas9 measured at 5 days posttransfection. Dotted line indicates the GFP-positive value of untreated cells.