BioDesign Research / 2021 / Article / Fig 2

Research Article

Phagocytosed Polyhedrin-Cytokine Cocrystal Nanoparticles Provide Sustained Secretion of Bioactive Cytokines from Macrophages

Figure 2

Characterization of professional phagocyte postphagocytosis of PODS proteins. (a) M0, M1, and M2 macrophages were incubated with PODS Empty or PODS FGF-10 (1 : 5 and 1 : 15) for 24 h in a 96-well TC plate. Subsequently, the medium was changed and viability of cells was measured 48 h after PODS uptake using a colorimetric assay (Orangu™, Cell Guidance Systems). The fold change in viability was calculated relative to unloaded macrophages of the same polarization state. Error bars represent the standard deviation of samples (). Significant differences to control (). (b) Medium of THP-1, M0, M1, and M2 was collected directly after polarization and tested for the presence of IL-6 by ELISA. Error bars represent the standard deviation of samples (). (c) The same cells were then washed and incubated with PODS FGF-2 for 24 h in a fresh full growth medium. The medium was then collected and analyzed for the presence of IL-6 by ELISA. Error bars represent the standard deviation of samples (). There was no significant difference between samples and controls.