BioDesign Research / 2021 / Article / Fig 3

Research Article

Phagocytosed Polyhedrin-Cytokine Cocrystal Nanoparticles Provide Sustained Secretion of Bioactive Cytokines from Macrophages

Figure 3

Mobility and chemotaxis of M0 cells loaded up with PODS. (a) Mobility of PODS IL-2 loaded macrophages. The mobility of empty and PODS IL-2-loaded macrophages (10 PODS/cell) in a full growth medium was monitored using a live cell imaging system. Images were taken every 2 min for 24 h and analyzed using the manual tracker of ImageJ and the chemotaxis and migration tool (Ibidi). 25 cells per condition were followed, and their migration tracks were visualized as a rose plot, in which each line represents the track of an individual cell over 24 hours. Track positions were normalized so that each track starts at the (0,0) coordinate. (b) Empty and PODS IL-2-loaded macrophages were transferred into Chemotaxis μ-Slides (Ibidi) and tested for their ability to follow a chemoattractant gradient generated from 0 to 10% BCS. Images were taken every 1 min for 12 h and analyzed using the manual tracker utility of ImageJ and the chemotaxis and migration tool (Ibidi). 26 cells per condition were followed, and their migration tracks were visualized as a rose plot, in which each line represents the track of an individual cell over 12 hours. Track positions were normalized so that each track starts at the (0,0) coordinate. Tracks of cells migrated towards the 10% BCS source are shown in black (up), whereas tracks of cells migrated away from the BCS source are shown in red (down).