BioDesign Research / 2021 / Article / Fig 4

Research Article

Phagocytosed Polyhedrin-Cytokine Cocrystal Nanoparticles Provide Sustained Secretion of Bioactive Cytokines from Macrophages

Figure 4

Directed migration of PODS eGFP loaded macrophages through 8 μm pores. (a) PODS eGFP-loaded macrophages (5 PODS/cell) in a serum-free medium were transferred into 24-well inserts with 8 μm pores. The bottom well was filled with either RPMI-1640 only (left) or RPMI-1640 supplemented with 10% FBS (right). The top images show to the bottom well-migrated cells in the brightfield channel, and the lower images show the corresponding fluorescent channel after 24 h of incubation. (b) PODS eGFP-loaded macrophages follow chemical signals from the melanoma cell line through 8 μm pores. PODS eGFP-loaded macrophages (5 PODS/cell) in full growth medium were transferred into 24-well inserts with 8 μm pores. The well was filled with either full growth medium (left) or medium that was conditioned with A375 cells for 3 days (right). The top images show the bottom well-migrated cells in the brightfield channel, and the lower images show the corresponding fluorescent channel after 24 h of incubation. The cells were imaged at a magnification of 10x, and the scale bar is 100 μm.