Phagocytosed Polyhedrin-Cytokine Cocrystal Nanoparticles Provide Sustained Secretion of Bioactive Cytokines from Macrophages
Release of IL-6 from PODS IL-6 loaded macrophages. (a) M0 was loaded with 5, 10, or 20 of either PODS IL-6 or PODS FGF-2 per cell (equivalent to ,, and PODS per 96-well). Cells were washed and incubated in full growth medium for 4 d. The medium was collected and tested for the presence of IL-6 in the medium. Error bars represent the standard deviation of samples (). IL-6 levels of controls were below background. (b) The same amounts of PODS IL-6 as before were spun down to the bottom of a 96-well plate and incubated for 4 d in full growth medium. The medium was collected, and levels of IL-6 were measured by ELISA. Error bars represent the standard deviation of samples ().