BioDesign Research / 2021 / Article / Fig 1

Research Article

Production of Volatile Moth Sex Pheromones in Transgenic Nicotiana benthamiana Plants

Figure 1

Stable and transient expression in Nicotiana benthamiana of the synthetic moth pheromone pathway. (a) Schematic view of the T-DNA construct used for transient expression, carrying the three transgenes AtrΔ11, HarFAR, and EaDAct, each under the control of the constitutive CaMV35s promoter and terminator, and the biosynthetic route of the moth pheromones. (b) GC/MS analysis of the volatile profile of N. benthamiana transiently expressing the transgenes (blue line) and a mock infiltrated plant with only P19 (red line). Peaks corresponding to the target insect pheromones are indicated with a label. Highlighted in red is the region of the (Z)-11-hexadecenal peak. (c) Schematic view of the T-DNA construct for the SxPv1.0 encoding the three transgenes and two selection markers. The two selection markers DsRed and NptII are highlighted in red and purple, respectively. (d) Pheromone content in SxPv1.0 T0 plants (numbered from 1 to 11). The diameter of each dot corresponds to the (Z)-11-hexadecenal level of each sample. Plants marked with a red cross died before seeds could be collected. (e) Overlapped chromatograms showing the volatile profile of a representative SxPv1.0 T0 plant (blue line) and a WT plant (red line).