BioDesign Research / 2021 / Article / Fig 7

Research Article

Production of Volatile Moth Sex Pheromones in Transgenic Nicotiana benthamiana Plants

Figure 7

Roadmap for future SxP versions. The bottlenecks identified in SxPv1.n serve as guidance for future iterations in the development of pheromone biofactories. Key improvements in a second generation (SxPv2.n) should include inducible expression of the pheromone pathway to circumvent growth penalties, ideally triggered by environmentally friendly agrochemicals. Additionally, it should increase emission rates, e.g., by coexpressing carrier proteins. Progress towards a third generation (SxPv3.n) would require the transfer of SxPv2.n tools to a related chassis with higher biomass, probably N. tabacum. Other improvements would involve the ability to produce different pheromone “programs” in the same plant, each one triggered by different chemicals, and the selective accumulation of pheromones in glandular trichomes to facilitate their release. Ideally, subsequent iterations (SxPv4.n) should incorporate, among others, the ability to respond directly to the presence of the target pest, as well as additional improvements in the chassis itself that facilitate its use as biosafe emitters in the field (e.g., nonflowering).