Overview of the phosphatase-less Calvin-Benson-Bassham cycle variant that was designed in this work. Dashed lines represent the phosphotransferase reactions that were added which replace the phosphatase reaction and transfer 2 phosphate groups to ribulose-5-phosphate (R5P). For simplification, reaction arrows can include multiple reactions and skip some metabolites. ADP, AMP, phosphate, water, and oxidized forms of electron carriers were also left out to improve clarity. Abbreviations: R15P: ribulose-1,5-bisphosphate; 3PG: 3-phosphoglycerate; 13BPG: 1,3-bisphosphoglycerate; GAP: glyceraldehyde-3-phosphate; FBP: fructose-1,6-bisphosphate; F6P: fructose-6-phosphate; S17BP: seduheptulose-1,7-bisphosphate; PPi: pyrophosphate; S7P: seduheptulose-7-phosphate; R5P: ribulose-5-phosphate.