BioDesign Research / 2021 / Article / Fig 6

Research Article

In-Depth Computational Analysis of Natural and Artificial Carbon Fixation Pathways

Figure 6

Comparison of the specific activity of natural and artificial carbon fixation pathways with the Enzyme Cost Minimization algorithm. The final product of choice was glyceraldehyde-3-phosphate in this case.. The concentrations of CO2 and HCO3- were assumed to not exceed 10 μM and 100 μM, respectively, corresponding approximately to air saturation. Pathway-specific activities with standard deviations and the full name of the main pathways and the connecting modules to transform their primary product to glyceraldehyde-3-phosphate. Pathway abbreviations: CBB(PTS): phosphatase-free CBB cycle; HP-bicycle(glyox): glyoxylate-producing subcycle of the 3-HP bicycle; PyrC: pyruvate carboxylase; OAAtoGAP: gluconeogenesis; b-OH-AspCycle: β-hydroxyaspartate cycle; AcCoAtoOAA(HP): acetyl-CoA to oxaloacetate-converting module derived from the 3-HP/4-HB cycle using either ADP- or AMP-producing CoA-ester synthases; rCC-AcCoAtoPyr: acetyl-CoA to pyruvate-converting module derived from the rCCC; GlyoxToOAA: glyoxylate- to oxaloacetate-converting module based on the Serine cycle; GlyoxToGAP(GCL/GDH): glyoxylate conversion by glyoxylate carboligase and glycerate dehydrogenase. The overall stoichiometries of all pathways are listed in supplementary Table S5.