BioDesign Research / 2022 / Article / Tab 5

Review Article

Design of Protein Segments and Peptides for Binding to Protein Targets

Table 5

Examples of de novo designed scaffolds used for binding to protein targets.

Designed scaffoldTargetResults

FoldamersMDM2IC50 as low as 24 nM [127]
FoldamersVitamin D receptorKd as low as 0.12 μM [127]
Terphenyl scaffoldsBcl-XLKi as low as 0.114 μM [128]
De novo coiled coilsMCL-1IC50 as low as 0.09±0.005 μM [130]
[3]-peptidegp41EC50 as low as 5.3±0.5 [134], one variant was later improved by near 7 folds through mutagenesis [135]
-peptidehDM2IC50 of 80.8 ± 3.2 nM [136], further improved to as low as 27.6 nM for MDM2 upon mutagenesis [137]
-hairpinHDM2IC50 values as low as 0.14±0.06 after optimization [129]
Designed peptide-peptoidTCF/-cateninBest binder had IC50 of 5.44 0.82 μM [133]
Miniproteins of ~65 residueSARS-COV25 pM after affinity maturation [13]