Making Use of Plant uORFs to Control Transgene Translation in Response to Pathogen Attack
Various uORFs combinations show different downstream repressing efficiencies. (a) Repression of GFP production by ACD11 uORF mutants. Photos were taken 48 hours post agro-infiltration. Illustration of the indicated plasmids was shown below. (b) Relative fluorescence in leaves expressing indicated plasmids. Relative fluorescence was calculated based on the formula shown in Figure 1(c) (;, Student’s -test, ). (c) Relative transcript accumulation levels of GFP. Transcript accumulation levels of GFP were analyzed by qRT-PCR with NbELF18 as internal reference. Bars represent standard errors from three biological replicates (;;,; Student’s -test). (d) Decreased GFP protein expression confirmed by Western blot assay. The GFP protein accumulation was detected by α-GFP antibody. Equal loading of each sample is indicated by the luciferase protein.