Making Use of Plant uORFs to Control Transgene Translation in Response to Pathogen Attack
uORFACD11-mediated translational repression is attenuated upon pathogen infection. (a) GFP accumulation after pathogen-related treatments. Confocal microscope images were taken 12 hours posttreatment. . (b) Mean fluorescence value of indicated leaves. The average GFP florescence densities were quantified per 100 pixels of 20 randomly selected cells (relative unit) using ImageJ. (c) Relative transcript accumulation levels of GFP. Transcript accumulation levels of GFP were analyzed by qRT-PCR with AtUBQ10 as internal reference. Bars represent standard errors from three biological replicates (;;,; Student’s -test). (d) GFP protein accumulation detected by Western blot assay. The α-GFP antibody was used to detect the expression of GFP protein. Equal loading of each sample is indicated by Ponceau staining. (e) GFP protein accumulation changes after P. capsici inoculation. The GFP protein accumulation was detected by α-GFP antibody. Equal loading of each sample is indicated by Ponceau staining.