BioDesign Research / 2022 / Article / Fig 5

Research Article

Making Use of Plant uORFs to Control Transgene Translation in Response to Pathogen Attack

Figure 5

Different uORFsACD11 cassettes lead to variable AtLecRK-VI.2 protein accumulation and pathogen resistance levels. (a) AtLecRK-VI.2 protein accumulation levels detected by Western blot assay. The α-FLAG antibody was used to detect the expression of AtLecRK-VI.2 protein. Equal loading of each sample is indicated by Ponceau staining. (b) Relative transcript accumulation levels of AtLecRK-VI.2. Transcript accumulation levels of AtLecRK-VI.2 were analyzed by qRT-PCR with NbELF18 as internal reference. Bars represent standard errors from three independent biological replicates (; ; , ; Student’s -test). (c) Expression of AtLecRK-VI.2 driven by ACD11 uORF-mutants provides variable levels of resistance to P. capsici infection in N. benthamiana. Lesion areas were calculated at 48 hpi. The result was calculated from three independent replicates with at least five leaves per replicate (; ; , , Student’s -test). (d) No pathogen resistance is induced by uORF1, uORF2, or uORF3. Leaves were stained by trypan blue and destained with ethanol. Photos were taken in white light and shown in (a). . The lesion areas were calculated from three independent experiments were shown below (; ; , , Student’s -test).