BioDesign Research / 2022 / Article / Fig 1

Review Article

Reflection on the Challenges, Accomplishments, and New Frontiers of Gene Drives

Figure 1

Illustration of CRISPR-based gene drive inheritance compared to natural Mendelian inheritance. Insects and other organisms typically have a 50% chance of passing an altered gene to their progeny (a). When inherited this way, the altered gene may not spread to many individuals in the population and will vanish after many generations. However, if the altered allele is linked to a CRISPR/Cas9 gene drive as shown in the schematic on the upper-right side of (b), the CRISPR gRNA will guide the Cas9 to the target site on the WT chromosome to make a double-strand break at the target site. The break will be repaired through HDR using the drive-containing chromosome as a template. This will result in the gRNA, the Cas9 drive, and the altered gene being copied into the homologous chromosome guaranteeing that it gets passed at higher rates (b).