Reflection on the Challenges, Accomplishments, and New Frontiers of Gene Drives
RNAi-based toxin-antidote gene drive systems developed.
Transposable element vector in which the maternal germline-specific bicoid promoter drives the expression of a polycistronic transcript encoding two microRNAs (miRNAs) designed to silence expression of the gene producing the toxin, myd88
The theoretical design relies on environmental cues that could be used to cause a population crash. The MEDEA will be given time to spread before the cue appears and result in cue-dependent suppression
Theoretical design (not tested experimentally on any species)
The designed system consisted of 2 genetic components: a zygotic toxin and maternal antidote that result in heterozygous progeny of wild-type (WT) mothers being unviable
UDMEL system consists of 2 constructs: UDMEL-1 comprises maternal toxin A and zygotic antidote B, and UDMEL-2 includes maternal toxin B and zygotic antidote A. Both toxins target essential genes for normal embryonic development
Medusa system consisted of four components: a maternally expressed X-linked toxin and a zygotically expressed Y-linked antidote, a zygotically expressed Y-linked toxin, and a zygotically expressed X-linked antidote