Imaging of genetically encoded calcium indicators. (a) Schematic of GCaMP family of calcium indicators. (b) Example fluorescence trace of a single neuron. (c) Different approaches to express calcium indicators in rodents. (d) Cell-type-specific expression of GECIs in cre-positive neurons. (e) Head-fixed imaging. (f) Imaging in freely-moving mice using head-mounted microscopes. (g) General calcium image analysis pipeline.