BME Frontiers / 2020 / Article / Tab 1

Review Article

From Neurons to Cognition: Technologies for Precise Recording of Neural Activity Underlying Behavior

Table 1

Detailed comparison of various technologies for recording neuronal activity in rodents.

Ca2+ imagingVoltage imagingElectrophysiology
1P-Ca1P-Ca-head mounted2P-Ca2P-Ca-head mounted1P-V2P-VMicrowire electrodesSilicon electrodesNext-gen probesFlexible electrodes

Typical # of neurons/units resolvable100-1000100-50098100-50031,10-10095~1044~1043~20 per probe115~100 per probe123~300 per probe129~15 per probe136
Typical temporal resolution5-25 Hz5-25 Hz5-25 Hz5-25 Hz0.5-1 kHz44,45,480.2-4 kHz43,68>1 kHz>1 kHz>1 kHz>1 kHz
Area of tissue damageBrain surfaceΦ 0.5-1 mm holeBrain surfaceΦ 1-2 mm holeBrain surfaceBrain surfaceΦ 0.2 mm hole<Φ 0.1 mm hole<Φ 0.1 mm hole<Φ 0.01 mm hole
Subcellular resolution?NoNoYes30Yes95NoYesNoNoNoNo
Freely moving behavior?NoYes98NoYes95NoNoYes115Yes123Yes129Yes136
Chronic recording?§YesYesYesYesYesYesNoNoNoYes134
Human applicable?NoNoNoNoNoNoNoYes128NoNo

While typical 2p microscopes can image field of views with a maximum of  mm, 2p mesoscopes are capable of imaging large field of views ( mm) resolving up to 3000 neurons simultaneously, though at lower temporal resolution (2 Hz)71. Temporal resolution for Ca2+ imaging is limited by slow dynamics of intracellular calcium and calcium indicators. Microscope setups are able to record at higher temporal resolution; however, accurate inference of neuronal spiking is only feasible up to a firing rate of ~25 Hz31, 106. The area of tissue damage depends on a targeted brain region. Areas stated for Ca2+ imaging assumes cortical imaging for 1P-Ca and 2P-Ca and deep brain imaging using GRIN lens implantations for head-mounted imaging. §Capability to long-term track individual identified neurons over multiple days.