Study protocol. Histotripsy ablation (either partial or entire tumor) was performed once the tumor measured a minimum of 5 mm in the largest diameter. The survival groups A, B, and C were survived and monitored weekly using MRI for up to 3 months post treatment or until the maximum tumor diameter exceeded 25 mm. The acute rats (group D) were euthanized immediately after ablation. For the partial ablation groups A and D, 50-75% of the tumor volume was ablated by histotripsy. In the entire tumor ablation group B, the entire tumor with additional margin was targeted for ablation. Animals in control group C received no treatment.