Multimodal nonlinear microscopy. (a) Spectral representation (top) of excitation wavelength and resultant emission wavelengths for THG and SHG. Corresponding Jablonski diagrams (bottom), where dashed lines represent virtual electronic states. Representative H&E, SHG, and SHG+THG images of breast tissues diagnosed as normal (first row), fibroadenoma (second row), and invasive lobular carcinoma (third row). (b) Simple scheme of multimodal microscope beam path for nonlinear imaging. Reproduced from Ref. [14
] published under CC BY 3.0, adapted from Ref. [28
] published under CC BY 4.0.