Cyborg and Bionic Systems / 2021 / Article / Fig 5

Research Article

Bioprinted Vascularized Mature Adipose Tissue with Collagen Microfibers for Soft Tissue Regeneration

Figure 5

Viability and vascularization assessment of the bioprinted adipose tissues. (a) Representative images of the cell viability for bioprinted tissues containing only mature adipocytes. (b) Representative images of the cell viability for bioprinted tissues containing the three types of cells (mature adipocytes, HUVEC, and ADSC). (c) Quantitation of the cell viability from the Live/Dead images (AD: only mature adipocytes; VASC: the three types of cells). (d, e) Cross-section and representative images of fluorescent immunostainings of the CD31 endothelial cell marker, Nile Red lipid staining for mature adipocytes, and Hoechst for nuclei on bioprinted tissues and in vivo. These results come from three independent experiments with 1-3 tissues per experiment and condition.