Bioprinted Vascularized Mature Adipose Tissue with Collagen Microfibers for Soft Tissue Regeneration
Mature adipocyte functionality assessment of the bioprinted adipose tissues. Monitoring of fatty acid uptake and accumulation was realized by the addition of a fluorescent fatty acid analog (4 μM BODIPY™ 500/510 C1, C12) following one hour of incubation with insulin induction, counterstained by Propidium Iodide (PI) for staining the dead cells. (a) Representative images of fatty acid uptake in tissues with only mature adipocytes. (b) Representative images of fatty acid uptake in vascularized adipose tissues containing the three types of cells. samples per condition were performed.