Schematic of recovery, harvesting, transferring, and layering cell sheet using the gelatin-coated plunger device. (a) Macroscopic view of gelatin-coated plunger-like device as a cell sheet manipulator. (b) Cell sheet was covered by the gelatin-coated device and incubated at 20°C. (c) Cell sheet was harvested by the device. (d) The harvested cell sheet was transferred to a new cell culture surface. (e) The harvested cell sheet was layered on another cell sheet. The images from reference [36
] (Z. Tang and T. Okano., Recent development of temperature-responsive surfaces and their application for cell sheet engineering, Regen Biomater, 2014, 1, pp. 91-102) are under a Creative Commons Attribution 4.0 International License (CC-BY-4.0).