Cyborg and Bionic Systems / 2021 / Article / Fig 5

Research Article

Pulsed Microfluid Force-Based On-Chip Modular Fabrication for Liver Lobule-Like 3D Cellular Models

Figure 5

Long-term coculture of the liver lobule-like 3D model. (a) Bright-field image of the liver lobule-like 3D model consists of an inner HepG2-laden PEGDA substructure and outer HUVEC-laden GelMA substructure. (b) Live/dead staining of the cells in the 3D model. (c) Evaluation of the cell viability of the liver lobule-like 3D model during in vitro culture using CCK-8. (d) Cell proliferation in the 3D model during in vitro culture. (e) Geometries of the cross-section of the liver lobule-like 3D model and a comparative example of a multilayered 3D with a traditional circular inner structure.