The Use of High-Throughput Phenotyping for Assessment of Heat Stress-Induced Changes in Arabidopsis
More pronounced reduction of the rosette size with increased length of heat treatment and mutation of hsp101. (a) Representative RGB, mask, and chlorophyll fluorescence images of WT and hsp101 rosettes of plants underwent 6 h heat stress treatment. The colour code depicted at the right represents the maximal quantum yield (QY max) from blue (low ) to red (high ). DAS: days after stress. (b) Comparisons of rosette size of nonstressed WT plants with 3 h, 6 h, and 9 h heat-stressed WT. (c) Comparisons of rosette size of 3 h, 6 h, and 9 h heat-stressed WT and hsp101. (d) Comparisons of QY max of nonstressed WT plants with 3 h, 6 h, and 9 h heat-stressed WT. (e) Comparisons of QY max of 3 h, 6 h, and 9 h heat-stressed WT and hsp101. Dashed lines and grey ribbons represent the of different plant groups ( varies between 17 and 20 for individual groups). The significance of the difference in the size of treated and nontreated WT (b, d) and hsp101 (c, e) plants each day during the imaging period was determined by Student’s -test, with a value below 0.05, 0.01, 0.001, and 0.0001 indicated with ,,, and , respectively.