The Use of High-Throughput Phenotyping for Assessment of Heat Stress-Induced Changes in Arabidopsis
Heat stress-induced changes in chlorophyll fluorescence show a dynamic profile. The comparison of the directly measured chlorophyll fluorescence traits 1 h after 6 h of heat stress treatment (45°C) application in (a) WT compared to WT nonstressed plants and (b) WT compared to hsp101 plants exposed to heat stress. The directly measured chlorophyll fluorescence traits were also measured at 1 day after stress (DAS) in (c) WT compared to WT nonstressed plants and (d) WT compared to hsp101 plants exposed to heat stress. The average of individual groups is represented by the black dot, while the measurements derived from individual plants are represented using transparent points. The and indicate minimal and maximal chlorophyll fluorescence measured at dark-adapted state, respectively, while ,, and at Lss1 to Lss6 represent minimal, steady-state, and maximal chlorophyll fluorescence, respectively, in a light-adapted state at light intensities of 95, 210, 320, 440, and 670 μmol m-2 s-1. The significance of the difference in chlorophyll fluorescence of treated and nontreated WT (a, c) and hsp101 (b, d) plants for individual parameters were determined by Student’s -test, with a value below 0.05, 0.01, 0.001, and 0.0001 indicated with ,,, and , respectively.