The Use of High-Throughput Phenotyping for Assessment of Heat Stress-Induced Changes in Arabidopsis
Heat stress transiently reduces photochemical and nonphotochemical quenching. (a) Comparison of photochemical quenching () of WT plants grown under control conditions and 3 h, 6 h, and 9 h of heat stress (45°C) treatment. (b) Comparison of heat-induced changes in in WT and hsp101 for plants exposed to 45°C for 3 h, 6 h, and 9 h. (d) Comparisons of nonphotochemical quenching (NPQ) of nonstressed WT plants with 3 h, 6 h, and 9 h heat-stressed WT (e) Comparisons of NPQ of 3 h, 6 h, and 9 h heat-stressed WT and hsp101. Dashed lines and grey ribbons represent the of different plant groups ( varies between 17 and 20 for individual groups). The significance of the difference in the size of treated and nontreated WT (a, c) and hsp101 (b, d) plants each day during the imaging period was determined by Student’s -test, with a value below 0.05, 0.01, 0.001, and 0.0001 indicated with ,,, and , respectively.