Research / 2018 / Article / Fig 5

Research Article

A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization

Figure 5

Rapid whole-cell labeling and mapping of HeLa cells upon 2 min incubation with activated alkynes. HeLa cells were quickly treated with three small fluorescent molecules, respectively, including TPA, alkyne-TPA, and the addition product of alkyne-TPA and n-butylamine.