Research / 2019 / Article / Fig 3

Research Article

Generating Giant Membrane Vesicles from Live Cells with Preserved Cellular Properties

Figure 3

Modification of GMVs with fluorescein-labeled ssDNA. Flow cytometry analysis and confocal image of GMVs incubated with (a) fluorescein-labeled PolyT10-lipid or (b) fluorescein-labeled ssDNA (L77). (c) GMVs encapsulating fluorescein-labeled L77 were stained with FM 4-64 dye. The Z-stack image (d) and 3D image (e) of a single giant vesicle are also shown. The overlay images of green fluorescence and red fluorescence demonstrate that fluorescein-labeled L77 was mainly located inside GMVs (f). Scale bar is 10 μm.