Characterization of Ablation Thresholds for 3D-Cultured Patient-Derived Glioma Stem Cells in Response to High-Frequency Irreversible Electroporation
Populations of patient-derived glioma stem cells are responsive to H-FIRE therapy at variable but lower thresholds than healthy astrocytes. (a) A visible lesion was created in collagen hydrogels seeded with cells. Electrode placement and ablation lesion outlined by dotted white line (b) Comparison of lesion areas shows three GSC populations have greater lesion sizes than healthy astrocytes and similar lesion sizes to bulk tumor cells and NSCs (c) GSC populations have a lower lethal threshold than healthy astrocytes and similar thresholds to U251 cells and NSCs when exposed to H-FIRE pulses. ,, and .