Characterization of Ablation Thresholds for 3D-Cultured Patient-Derived Glioma Stem Cells in Response to High-Frequency Irreversible Electroporation
Cells seeded in hyaluronic acid hydrogels exhibit more uniform morphologies compared to collagen-seeded cells. (a) Confocal images of cells seeded in HA hydrogels show a rounded and compact cell morphology regardless of cell type. (b) Cells seeded in HA have similar cell areas (p > 0.05) while malignant cells have larger nuclear areas than healthy cells (p < 0.01). (c) GSCs and U251 cells exhibit a larger NCR than NHAs: ;. (d) All three cell types exhibit similar circularity (p > 0.05) and aspect ratios (p > 0.05).