Comprehensive Flow Cytometry Analysis of PEI-Based Transfections for Virus-Like Particle Production

<div class="floats-full-page floats-full-page-figure"><h2>Figure 3</h2><div class="floats-full-page-caption"><div>Analysis of transfections based on ChikVLP staining vs. plasmid fluorescence. (a) Day 3 flow cytometry cell population heat map for each sample tested. (b) Flow cytometry results of the 25<svg height="6.1673pt" id="M21" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -5.96091 5.50181 6.1673" width="5.50181pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M391 364C391 409 353 448 295 448C249 448 198 426 152 393C65 331 23 225 23 139C23 14 96 -12 146 -12C198 -12 280 9 367 101L351 124C300 78 242 48 194 48C129 48 109 107 109 162V191C208 213 391 266 391 364ZM313 350C313 305 268 261 113 223C132 334 187 381 217 398C227 404 244 405 261 405C290 405 313 385 313 350Z"></path></g></svg>6 cells/mL sample grouped into different expression (high-, medium-, low-, and non-) staining fractions and plotted against the control (-) Cy5 transfection sample. (c) Plasmid MFI of each VLP staining fraction for each transfection cell density (bar graph). The percentages (%) listed above bars represent the cell population fraction within that staining classification.</div></div><div class="floats-full-page-content"><table class="figure-group"><tr class="fig-image"><td><object data="https://d31iiq90dwlpfy.cloudfront.net/articles/research/volume-2020/1387402/figures/1387402.fig.003.svgz" name="1387402.fig.003" type="image/svg+xml"></object></td></tr></table></div></div>

Figure 3 | Comprehensive Flow Cytometry Analysis of PEI-Based Transfections for Virus-Like Particle Production

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Figure 3: Comprehensive Flow Cytometry Analysis of PEI-Based Transfections for Virus-Like Particle Production

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Figure 3

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<p>The open access journal <em>Research</em>, published in association with CAST, publishes innovative, wide-ranging research in life sciences, physical sciences, engineering and applied science.</p>


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The open access journal Research, published in association with CAST, publishes innovative, wide-ranging research in life sciences, physical sciences, engineering and applied science.