Comprehensive Flow Cytometry Analysis of PEI-Based Transfections for Virus-Like Particle Production

Analysis of transfections based on ChikVLP staining vs. plasmid fluorescence. (a) Day 3 flow cytometry cell population heat map for each sample tested. (b) Flow cytometry results of the 256 cells/mL sample grouped into different expression (high-, medium-, low-, and non-) staining fractions and plotted against the control (-) Cy5 transfection sample. (c) Plasmid MFI of each VLP staining fraction for each transfection cell density (bar graph). The percentages (%) listed above bars represent the cell population fraction within that staining classification.