Research / 2020 / Article / Fig 6

Review Article

The Strategies of Pathogen-Oriented Therapy on Circumventing Antimicrobial Resistance

Figure 6

RGN constructs delivered by bacteriophage particles (ΦRGN) exhibit efficient and specific antimicrobial effects against strains harboring plasmid or chromosomal target sequences. (a) Bacteriophage-delivered RGN constructs differentially affect host cell physiology in a sequence-dependent manner. (b) Treatment of EMG2 wild-type (WT) or EMG2 containing native resistance plasmids, pNDM-1 (encoding blaNDM-1), or pSHV-18 (encoding blaSHV-18), with SM buffer, ΦRGNndm-1, ΦRGNshv-18, or multiplexed ΦRGNndm-1/shv-18 at a multiplicity of infection (MOI) ~20 showed sequence-dependent cytotoxicity as evidenced by a strain-specific reduction in viable cell counts (). CFU: colony-forming units. (c) E. coli EMG2 WT or EMG2 gyrAD87G populations were treated with SM buffer, ΦRGNndm-1, or ΦRGNgyrAD87G at MOI ~20, and viable cells were determined by plating onto Luria-Bertani agar () [231]. Copyright © 2014, Springer Nature.