Research / 2020 / Article / Fig 9

Review Article

The Strategies of Pathogen-Oriented Therapy on Circumventing Antimicrobial Resistance

Figure 9

Schematic diagram of constructing a functional probiotic strain and its activity against P. aeruginosa infection. (a) Vector-host system created in the E. coli Nissle strain by auxotrophic complementation to stabilize plasmid retention. (b) P. aeruginosa was cocultured with engineered EcN for 20 h to evaluate its effect on biofilm formation. (c) The survival rate of each treatment group was quantified until all the nematodes in the infection group had died (96 h). (d) Evaluation of the engineered probiotic strain in a mouse infection model. (e) Prophylactic activity of engineered EcN against P. aeruginosa infection [264]. Copyright © 2017, Springer Nature.