Construction of Dual-Biofunctionalized Chitosan/Collagen Scaffolds for Simultaneous Neovascularization and Nerve Regeneration
Evaluation of the rat sciatic nerve using immunohistochemistry after scaffold implantation for 14 days. (a) Immunohistochemistry staining of NF200 (neurofilament200, red), CD34 (hematopoietic progenitor cell antigen, green), s100β (red), and Hoechst 33342 (blue) of the longitudinal sections of the regenerating nerve harvested at 14 d after bridging a 10 mm rat sciatic nerve gap with a plain CC scaffold and a dual-biofunctionalized scaffold with IKVAV/VEGF, respectively. The right images are the magnifications of the boxed areas. Left scale and right scale . (b–d) Histograms comparing (b) the migration of SCs, (c) the length of regenerating nerve fibers, and (d) the density of blood vessels regenerated in the scaffolds at 14 d after surgery (). And (e, f) comparison of compound muscle action potential (CMAP) amplitudes and (g, h) the wet weight ratio of the gastrocnemius and anterior tibialis muscles. Data are expressed as . versus control group.