Research Article | Open Access
Zonghai Sheng, Yaxi Li, Dehong Hu, Tianliang Min, Duyang Gao, Jen-Shyang Ni, Pengfei Zhang, Yuenan Wang, Xin Liu, Kai Li, Hairong Zheng, Ben Zhong Tang, "Centimeter-Deep NIR-II Fluorescence Imaging with Nontoxic AIE Probes in Nonhuman Primates", Research, vol. 2020, Article ID 4074593, 14 pages, 2020. https://doi.org/10.34133/2020/4074593
Centimeter-Deep NIR-II Fluorescence Imaging with Nontoxic AIE Probes in Nonhuman Primates
Fluorescence probes with aggregation-induced emission (AIE) characteristics are of great importance in biomedical imaging with superior spatial and temporal resolution. However, the lack of toxicity studies and deep tissue imaging in nonhuman primates hinders their clinical translation. Here, we report the blood chemistry and histological analysis in nonhuman primates treated with AIE probes over tenfold of an intravenous dose of clinically used indocyanine green (ICG) during a study period of 36 days to demonstrate AIE probes are nontoxic. Furthermore, through bright and nontoxic AIE probes and fluorescence imaging in the second window (NIR-II, 1,000–1,700 nm), we achieve an unprecedented 1.5-centimeter-deep vascular imaging in nonhuman primates, breaking the current limitation of millimeter-deep NIR-II fluorescence imaging. Our important findings, i.e., nontoxic features of AIE probes and centimeter-deep NIR-II vascular imaging in nonhuman primates, may facilitate successful translation of AIE probes in clinical trials.
Fluorescence imaging with organic molecules as probes has become an essential tool for bench researchers and clinicians interested in understanding disease mechanism or improving human health due to excellent spatial and temporal resolution [1–3]. However, the imaging sensitivity and spatial resolution can be generally weakened due to the aggregation-caused quenching (ACQ) of fluorescence probes at high concentrations or condensed states . Fortunately, twenty years ago, our team discovered a specific class of organic molecules emitting bright fluorescence even in the aggregated state; this phenomenon of fluorescence emission in the aggregated state is termed as aggregation-induced emission (AIE) . Since then, numerous AIE molecules have been synthesized as fluorescence probes for biomedical imaging research [6–10]. AIE probes exhibit excellent biocompatibility, high quantum yield (QY), tunable emission wavelength, large stoke shift, and great optical stability, enabling their use in next-generation fluorescence materials for biomedical imaging [11, 12]. Recently, our team and others utilized the bandgap engineering strategy to further extend the emission wavelength of AIE probes from the first near-infrared (650–950 nm, NIR-I) to the second near-infrared (1,000–1,700 nm, NIR-II) window and achieve deeper tissue imaging [13–18]. NIR-II fluorescence imaging with existing probes including some AIE probes has already demonstrated the ability of millimeter-deep tissue imaging in the mouse models through suppressed scatters of photons in tissue and diminished autofluorescence background [19–24]. However, challenges remain in realizing the clinical translation of AIE probes and imaging depths greater than the current limitation of millimeters in the NIR-II window.
Recently, Carr et al. reported NIR-II fluorescence imaging with the clinically approved contrast agent indocyanine green (ICG) . This was followed by the first clinical trial which was performed for NIR-II fluorescence imaging-guided liver tumor surgery with ICG as the contrast agent based on its reliable safety and extensive practice . The successful clinical implementation of ICG inspired us to focus on toxicity studies of AIE probes and the development of unprecedented centimeter-deep in vivo imaging application in the NIR-II window using these probes. Nonhuman primate studies are known to facilitate clinical trials because of the evolutionary relationship between nonhuman primates and humans [27, 28]. Previous studies in nonhuman primates have reported toxicity evaluation of fluorescence probes, including quantum dots , silicon nanoparticles , gold nanoparticles , and nanodiamonds . These studies have enhanced our understanding on the toxicity of fluorescence probes, providing significant implications for the studies of novel AIE probes [33–35]. Therefore, we speculated that preclinical studies in a nonhuman primate could facilitate in-depth understanding on the toxicity of synthetic AIE probes. Furthermore, preclinical trials involving nonhuman primates involve lower risk and reduced cost compared to human clinical trials and must be performed before trials in human. Moreover, we sought to increase NIR-II fluorescence imaging depth from millimeter to centimeter level. The bright AIE characteristics and NIR-II imaging window could facilitate deeper tissue imaging in nonhuman primates.
Here, we report the toxicity profile of AIE probes with high QY in three cynomolgus monkeys, one of the important nonhuman primates, over a study period of 36 days following the intravenous (IV) injection of 16 mg kg-1 AIE probes. One of them was subsequently euthanized for ex vivo histological analysis, and the other two were observed for further ten months. In vivo hematological and tissue compatibility analysis revealed that AIE probes are highly biocompatible. These bright and nontoxic AIE probes were successfully applied in NIR-II fluorescence imaging of the blood vessels and lymph nodes, achieving unprecedented 1.5-centimeter depth and high resolution in nonhuman primates. Our pilot study demonstrated ample clinical potential of nontoxic AIE probes in noninvasive biomedical imaging.
2. Results and Discussion
2.1. Synthesis and Characterization of AIE Probes
A typical NIR-II AIE molecule shown in Figure 1(a), 4,7-di(4-(4-octylthiophen-2-yl)-N,N-diphenylaniline)-benzo[1,2-c:4,5-c]bis([1,2,5]thiadiazole) (TTB) with a donor-acceptor-donor structure, was designed and synthesized by a two-step chemical reaction, and the products were subsequently characterized (Fig. S1–S7). The AIE feature of the as-prepared TTB molecule was evaluated by adding different amounts of tetrahydrofuran in water (Figure 1(b)). The results suggested that the fluorescence intensity of TTB gradually decreased with the rising water volume fraction () from 0 to 40 vol% and then increased when was further raised from 40 to 90 vol%, confirming the AIE signature . The TTB was demonstrated to be a NIR-II fluorophore due to the maximum fluorescence emission at 1050 nm (Figure 1(b)). To endow TTB with water solubility and enhanced brightness, DSPE-PEG2000, an amphiphilic polymer matrix and a type of clinically used excipient, was adopted to encapsulate numerous TTB molecules to form AIE probes (Figure 1(a)). The whole process is facile and efficient for large-scale fabrication without sophisticated equipment, supporting its potential for approval in clinical studies and applications. The produced AIE probes exhibited a spherical morphology with an average diameter of 35 nm and excellent monodispersity (Figure 1(c)). Moreover, AIE probes readily dispersed in the buffer solution without aggregations for more than six months (Fig. S8). They had a maximum absorption peak at 728 nm, an emission peak at 1050 nm, and the emission tail at 1350 nm, indicating representative NIR-II probes (Figure 1(d)). The fluorescence QY was ~10%, which was higher than most of the NIR-II fluorophores reported previously using IR-26 dye as a reference (Table S1 and Fig. S9). The excellent photostability of AIE probes in PBS was observed under continuous exposure of natural light for one month (Fig. S10). Furthermore, Cell Counting Kit (CCK-8) assay was applied for evaluating the cytotoxicity of AIE probes in endothelial cells of the human umbilical vein (Figure 1(e)). The assessment revealed low cytotoxicity of the AIE probes, as the cell viability remained above 95% after incubation with 200 μg mL-1 of AIE probes for 24 hours, suggesting low cytotoxicity. We further assessed the blood compatibility of AIE probes in cynomolgus monkeys using hemolytic analysis (Figure 1(f)). The hemolytic rate of blood samples incubated with AIE probes of 0 μg mL-1, 25 μg mL-1, 50 μg mL-1, 100 μg mL-1, 200 μg mL-1, and 400 μg mL-1 AIE probes for 24 hours was below 5%, indicating its excellent in vitro biocompatibility. These results suggested that the uniform, bright, stable, and in vitro biocompatible AIE probes designed in the present study could be an excellent candidate for NIR-II fluorescence imaging.
2.2. In Vivo Toxicity Evaluation in Cynomolgus Monkeys
Before performing in vivo imaging, we designed a dose escalation experiment using three dose schemes (1, 5, and 10 mg kg-1) over 35 days to further evaluate in vivo biocompatibility in three healthy adult cynomolgus monkeys (Figure 2(a)). No IV injection of AIE probes in cynomolgus monkeys has been reported to our knowledge. Therefore, the escalating of IV injection dose of AIE probes could effectively investigate the safety of the monkeys and detect the tolerated ability of AIE probes. The cumulative IV dose reaches 16 mg kg-1 (equivalent IV dose of an adult human ~5.3 mg kg-1, ), which was more than tenfold of the IV dose of ICG (0.5 mg kg-1) for NIR-II fluorescence imaging in clinical trials [25, 26]. Therefore, the designed dose-escalating experiment could be used to evaluate the tolerance of as-prepared AIE probes in cynomolgus monkeys. Before IV injection of AIE probes, physiological parameters and blood chemistry of three cynomolgus monkeys were measured as the control group. This experiment design can not only greatly minimize the error caused by individual differences but also potentially reduce the sample size of experimental monkeys. After IV injection of AIE probes with the dosage escalation of 1, 5 mg kg-1, and 10 mg kg-1, the body weight of the monkeys showed no fluctuation, indicating minimal systemic effects (Fig. S11). Similarly, there was no change in body temperatures during the test (Fig. S12). No statistically significant differences in daily behavioral patterns (eating, drinking, sleeping, and activity), neurological status, and physical features were observed (data not shown). Next, a complete blood count was conducted at regular intervals to investigate the immune response to AIE probes (Figures 2(d)–2(q)). Results showed no sharp fluctuation in the 14 hematological indicators compared with the control and reference values, indicating no acute toxicity. Similarly, serum biochemistry assays revealed that 10 indicators of liver function (TP, A/G, ALB, ALP, ALT, AST, DBIL, GGT, GLOB, and TBIL) and 2 indicators of kidney function (CREA and UREA) were normal throughout the examination period (Figures 2(p)–2(z-1)), suggesting no sign of liver and kidney injuries. At the end of the 36-day postinjection period, one cynomolgus monkey was randomly selected and euthanized, and its heart, liver, spleen, lung, kidney, brain, muscle, and lymph nodes were removed for histological analysis (Figure 3). Two clinical pathologists, both unaware of the AIE probe treatment, analyzed the tissue sections and found no abnormality in the myocardial fibers, no signs of inflammatory response in the liver, no pulmonary fibrosis in the lung, nor necrosis in all histological samples, and clear structure of the glomerular. These results provided preliminary evidence that AIE probes, with a cumulative IV dose of 16 mg kg-1, did not induce acute toxicity in cynomolgus monkeys during the experimental period. Moreover, two other AIE probe-treated cynomolgus monkeys have been raised for more than ten months without any adverse response.
2.3. In Vivo Biodistribution and Metabolism of AIE Probes
During the experiments, we detected fluorescence signals in fecal samples at different time points (Fig. S13), demonstrating that AIE probes could be gradually excreted from the body through feces. The clearance mechanism of AIE probes in vivo was further investigated to analyze their biodistribution and metabolism by ex vivo fluorescence imaging. The results showed that AIE probes were predominantly accumulated in the liver, spleen, and lymph nodes (Fig. S14). Conversely, there was no fluorescence signal observed in the muscle, stomach, brain, heart, intestine, kidney, and lung (Fig. S15), which suggested that AIE probes, like other nanoparticles with similar size and surface properties, were primarily recognized by the reticuloendothelial system . Although most AIE probes were accumulated in the liver, hepatic function remained normal during our experimental period, further demonstrating no evidence of acute toxicity. We will optimize the design of AIE probes and polymer matrix to improve their in vivo clearance efficiency in the future.
2.4. In Vivo NIR-II Fluorescence Perfusion Imaging
To exploit perfusion imaging of AIE probes, we employed a commercial NIR-II imaging system with an ordinary InGaAs camera, an 808 nm excitation laser (30 mW cm-2), and a 1250 nm long-pass filter to acquire vasculature information in a cynomolgus monkey. Shortly after the IV injection of AIE probes (dose: 2 mg kg-1), we collected video-rate fluorescence images to record vascular perfusion at 5 frames per second (Supplementary Video 1, Fig. S16). Even though our imaging speed of the camera was much slower than that of previously reported liquid nitrogen-cooled InGaAs camera , yet, we still achieved clear images of vascular perfusion due to the contrast enhancement of bright AIE probes in the cynomolgus monkey. Within the 138.5 s postinjection period, the AIE probes had fully perfused into the arteries and veins of the forearm and markedly outlined the superficial vasculature (Figure 4(a)). Moreover, this vascular perfusion imaging can also be performed in the absence of depilation (Figure 4(b) and Fig. S17), which was evident of excellent imaging quality with suppressed scatter and absorption of a photon and diminished autofluorescence background in the NIR-II window . This perfusion imaging can also be applied to visualize the capillaries in the scalp of monkeys noninvasively (Figure 4(c)). The diameter of the arteries at representative locations ranged from 0.4 mm to 3.1 mm based on the Gaussian-fitting full width at half maximum (FWHM) of the cross-sectional profiles (Figures 4(e)–4(h)). In addition to NIR-II fluorescence angiography, we visualized the axillary lymph nodes of the cynomolgus monkey using AIE probes after 5 min of subcutaneous injection (0.5 mL and 1 mg mL-1). The size of the axillary lymph nodes was (dashed red circle in Figure 4(d)), which was consistent with the in vitro data (Fig. S14). This result demonstrated great clinical potential of AIE probes in NIR-II fluorescence imaging-guided sentinel lymph node biopsy for cancer diagnosis and treatment [39, 40].
2.5. In Vivo Centimeter-Deep Fluorescence Vascular Imaging
Vascular imaging in deep tissue is critical for the diagnosis of cardiovascular diseases and tumors . Following the successful demonstration of AIE probes in superficial tissue with NIR-II imaging, we conducted a more challenging centimeter-penetration vascular imaging in the cynomolgus monkey (Figures 5(a)–5(d)). Here, we purposedly selected the axillary artery of the cynomolgus monkey to perform the NIR-II fluorescence imaging. Prior to AIE probe injection, the depth and size of the axillary artery were measured using ultrasonic color Doppler imaging, confirming the artery located ~1.5 cm beneath the skin with a diameter of 2.4 mm (Figure 5(f)). We chose the bifurcation of the axillary artery as the landmark (shown as the red arrow in Figure 5(c)) for both ultrasound and fluorescence imaging. After the IV injection of AIE probes (2 mg kg-1), we recorded the video-rate NIR-II fluorescence vascular imaging, illustrating the stationary 1.5 cm-deep axillary artery and the moveable superficial veins while pushing the skin (Supplementary Video 2). By measuring the Gaussian-fitting FWHM of the cross-sectional profiles, we observed no change in the position of the deep arteries while the superficial veins gradually moved away from the skin pushing direction (Figure 5(e)). The imaging signal-to-background ratio of the deep arteries increased from 4.8 to 6.5 due to skin lifting effect. The apparent diameter of the artery was (Figure 5(e)), which was consistent with the ultrasound measurement. This indicated that we achieved noninvasive vascular imaging at 1.5 cm depth in nonhuman primates using the AIE probes for the first time.
In conclusion, cynomolgus monkeys were IV injected with a cumulative dose of 16 mg kg-1 of designed AIE probes and survived without any adverse response. All measured physiological parameters and hematological markers in treated cynomolgus monkeys were in the normal range, and the histological analysis of the major organs did not reveal any acute toxicity. The AIE probes trapped in the liver, spleen, and lymphatic system could be excreted through feces. The findings indicated that the organic AIE probes can be considered safe for nonhuman primates on the timescale of our experiment. Furthermore, through NIR-II fluorescence imaging with bright AIE probes, we achieved the unprecedented 1.5 cm-deep vascular imaging with high resolution and high contrast. These two distinguished features of NIR-II AIE probes, nontoxic and centimeter-deep fluorescence imaging, enable them to become a promising fluorophore candidate for fluorophores for angiography and lymphadenopathy. Therefore, our preliminary findings in this study could pave the way to promote clinical translation of NIR-II AIE probes in human clinical trials in the near future.
4. Materials and Methods
4.1. Materials and Characterization
All reagents were obtained commercially and used without further purification, except tetrahydrofuran (THF), dichloromethane (DCM), and toluene, which were purified before use. All air- and moisture-sensitive reactions were carried out in flame-dried glassware under argon protection. 1H (400 MHz) and 13C (100 MHz) NMR spectra were recorded on Bruker AV400 spectrometers (Bruker, Billerica, MA, USA). 1H NMR and 13C NMR spectra used tetramethylsilane (TMS) as an internal standard in CDCl3. High-resolution mass spectra were measured using Q-Exactive with Dionex Ultimate 3000 (Thermo Fisher Scientific, Waltham, MA, USA). UV-Vis-NIR absorption spectra were collected with a Shimadzu UV-2600 spectrophotometer. Fluorescence spectra were measured with a fluorescence spectrometer (F900, Edinburgh Instruments Ltd.). The NIR-II fluorescence imaging experiments were conducted by using NIR-OPTICS Series III 900/1700 small animal imaging system (Suzhou NIR-OPTICS Co., Ltd., China). Ultrasonic color Doppler imaging was performed using the Resona 7 ultrasound system with a L11-3U ultrasonic probe (Mindray Medical International Co., Ltd., China).
4.2. Synthesis of 4-(4-Octylthiophen-2-yl)-N,N-diphenylaniline 2
A solution of 3-octylthiophene 1 (1.96 g, 10.0 mmol) in THF (40 mL) was dropwise added with 2.5 Mn-BuLi (4.8 mL, 12.0 mmol) at -78°C. The mixture was stirred for 3 h under nitrogen protection. Then, SnBu3Cl (6.51 g, 20 mmol) was added to the reaction mixture and stirred overnight at room temperature. The mixture was quenched with water and washed with ethyl acetate. The combined organic layer was dried over anhydrous Na2SO4 and concentrated. The residual was dissolved in toluene as 1 M solution. The 1 M solution (9.6 mL) was added with 4-bromo-N,N-diphenylaniline (1.56 g, 8 mmol) and Pd (PPh3)4 (0.092 g, 0.08 mmol) and dissolved in toluene (80 mL) under nitrogen protection. After refluxing for 24 h and then cooling to room temperature, the mixture was quenched with water and washed with ethyl acetate. The combined organic phase was dried over anhydrous Na2SO4 and evaporated in vacuo. The crude product was subjected to column chromatography on silica gel with PE/EA 20 : 1 to 2 as a light yellow oil (2.36 g, 67%). 1H NMR (400 MHz, CDCl3) 7.45-7.43 (m, 2H), 7.27-7.25 (m, 4H), 7.12-7.01 (m, 9H), 6.79 (d, , 1H), 2.59 (t, , 2H), 1.71-1.66 (m, 2H), 1.43-1.32 (m, 10H), 0.93 (t, , 3H). 13C NMR (100 MHz, CDCl3) 147.60, 147.09, 144.26, 143.80, 129.30, 129.21, 128.99, 126.53, 124.41, 124.19, 123.89, 123.73, 122.98, 118.72, 31.92, 30.68, 30.48, 29.47, 29.37, 29.30, 27.88, 26.87, 22.70, 17.55, 14.13, 13.62. HRMS (ESI): [M+H+] calculated for C30H34NS 440.2406; found: 440.24050.
4.3. Synthesis of TTB
A solution of 2 (2.20 g, 5.0 mmol) in THF (20 mL) was dropwise added with Mn-BuLi (2.4 mL, 6.0 mmol) at -78°C. The mixture was stirred for 3 h under nitrogen protection. Then, the reaction mixture was added with SnBu3Cl (3.26 g, 10 mmol) and stirred overnight at room temperature. The mixture was quenched with water and washed with ethyl acetate. The combined organic layer was dried over anhydrous Na2SO4 and concentrated. The residual was solved in toluene as 1 M solution. The 1 M solution (2.1 mL, 2.3 equiv.) was added with 4,7-dibromobenzo[1,2-c:4,5-c]bis([1,2,5]thiadiazole) BBTD (0.307 g, 0.9 mmol) and beta-4-platinum (0.100 g, 0.09 mmol, 0.1 equiv.) and dissolved in toluene (5 mL) under nitrogen protection. After refluxing for 24 h and then cooling to room temperature, the mixture was quenched with 1 M KF aqueous solution and washed with ethyl acetate. The combined organic phase was dried over anhydrous Na2SO4 and evaporated. The crude product was subjected to column chromatography on silica gel with PE/EA 10 : 1 to TTB as a black green solid (350 mg, 47%). 1H NMR (400 MHz, CDCl3) 7.57 (d, , 4H), 7.36 (s, 2H), 7.30-7.26 (m, 9H), 7.15-7.03 (m, 15H), 2.58 (t, , 4H), 1.63-1.61 (m, 8H), 1.34-1.26 (m, 8H), 1.18-1.10 (m, 8H), 0.81 (t, , 6H). 13C NMR (100 MHz, CDCl3) 153.24, 147.60, 147.45, 147.00, 145.68, 129.36, 128.18, 126.78, 124.80, 124.67, 123.39, 123.22, 116.08, 31.79, 30.51, 30.35, 29.73, 29.46, 29.31, 29.16, 22.64, 14.12. HRMS (ESI): [M+] calculated for C66H64N6S4 1068.40686; found: 1068.40698.
4.4. NIR-II QY Test
The NIR-II fluorescence QY of the TTB-based AIE probes was measured according to the previous reports . Generally, the absorption and emission spectra of AIE probes and IR-26 dye with five different concentrations at below OD 0.1 at 808 nm were measured, respectively. The integrated fluorescence (808 nm excitation) was plotted against absorbance for both AIE probes and IR-26 dye. The comparison of the slopes led to the measurement of the QD of AIE probes according to the following equation: where is the QY of AIE probes, is the QY of IR-26 (0.5%), and and are the refractive indices of AIE probes and IR-26.
4.5. In Vitro Cellular Studies
Human umbilical vein endothelial cells were cultured at 37°C in Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum and 1% penicillin/streptomycin with a humidified environment containing 5% CO2. Human umbilical vein endothelial cells ( cells per well) were seeded in 96-well plates and incubated with various concentrations of AIE probes (0 μg mL-1, 3.125 μg mL-1, 6.25 μg mL-1, 12.5 μg mL-1, 25 μg mL-1, 50 μg mL-1, 100 μg mL-1, and 200 μg mL-1) for 24 h. The whole blood of monkey was collected, centrifuged (1500 rpm, 3 min) to separate the red blood cells, and washed (1500 rpm, 3 min) with PBS three times. 10% RBC (, in PBS) was incubated with AIE probes (0 μg mL-1, 25 μg mL-1, 50 μg mL-1, 100 μg mL-1, 150 μg mL-1, 200 μg mL-1, 300 μg mL-1, and 400 μg mL−1) at 37°C for 3 h, respectively. After centrifugation, the supernatant of the suspensions was collected and analyzed by a UV-vis spectrometer at 541 nm.
4.6. Animal Studies
Four cynomolgus monkeys (male, 3–4 kg, 4–5 years) were obtained from the Huazhen Laboratory Animal Breeding Centre (Conghua, Guangzhou, China). All animal experiment procedures were performed in compliance with the Institutional Animal Care and Use Committee of Huazhen Laboratory Animal Breeding Centre, China (no. HZ-AEC-TORM-051). The cynomolgus monkeys were housed individually in stainless steel cages (20–22°C, 40–60% relative humidity, 12-hour light-dark cycle, 15 air changes per hour) and fed a commercial monkey diet. Fresh fruits were also supplemented, and sufficient water was available. Research staff inspected the monkeys three times each day. Cynomolgus monkeys remained healthy and active, and food intake and body weight were normal. After collecting fasting blood samples, AIE probes (1 mg mL-1) dispersed in 0.9% NaCl were filter-sterilized before injection. Blood samples for the analysis of clinical chemistry and clinical hematology analyses were collected on days 0, 1, 2, 4, and 7 of each injection cycle. Body mass, temperature, appearance, and exploratory behavior were recorded at the same time. The clinical chemistry and clinical hematology analyses were carried out on the pocH-100ivD fully automated hematology analyzer (Sysmex Corporation, Kobe, Japan).
4.7. Histological Analysis
A randomly selected cynomolgus monkey was euthanized at the end of 36 days postinjection of AIE probes. The heart, liver, spleen, lungs, kidneys, colon, muscle, lymph nodes, and brain were collected and fixed with 4% paraformaldehyde. The tissue slices were stained with hematoxylin and eosin and observed on the digital (Pannoramic Desk; 3D Histech, Budapest, Hungary). Tissue sections were examined by two independent clinical pathologists.
4.8. In Vivo NIR-II Fluorescence Imaging
NIR-II fluorescence imaging was performed using NIR-OPTICS Series III 900/1700 small animal imaging system. The fluorescence imaging parameters were 808 nm laser with a power density of 30 mW cm-2, 1250 nm long-pass filter, and 200 ms exposure time using a pixel two-dimensional InGaAs/SWIR camera (Photonic Science, UK), with an intravenous dose of 2 mg kg-1.
Conflicts of Interest
The authors declare that there is no conflict of interest regarding the publication of this article.
Z. Sheng, H. Zheng, K. Li, and B. Tang designed the research. Z. Sheng, D. Hu, D. Gao, T. Min, J. Ni, X. Li, Y. Wang, and P. Zhang performed the research. Z. Sheng and Y. Wang analyzed the data. Z. Sheng, H. Zheng, Y. Wang, K. Li, and B. Tang cowrote the paper.
This work is supported by the National Key Research and Development Program of China (Scientific and Technological Innovation Cooperation of Mainland and Macao) (2017YFE0120000), the Natural Science Foundation of China (91859117, 81771906, 81901812, 81527901, and 31870991), the CAS Key Laboratory of Health Informatics (2011DP173015), the Guangdong Innovative and Entrepreneurial Research Team Program (2019ZT08Y191), the Science and Technology Innovation Fund of Shenzhen (JCYJ20170818161918918 and JCYJ20190812163614809), the Shenzhen Key Laboratory of Ultrasound Imaging and Therapy (ZDSYS201802061806314), and the China Postdoctoral Science Foundation (2019M653129).
Supplementary 1. Supplementary 1. Fig. S1: synthetic route of the TTB molecular. Fig. S2: 1H NMR spectrum of 2 in CDCl3. Fig. S3: 13C NMR spectrum of 2 in CDCl3. Fig. S4: high-resolution mass spectrum of 2. Fig. S5: 1H NMR spectrum of TTB in CDCl3. Fig. S6: 13C NMR spectrum of TTB in CDCl3. Fig. S7: high-resolution mass spectrum of TTB. Fig. S8: the particle size of AIE probes over storage time. Fig. S9: measurement of the quantum yield of AIE probes using IR26 as reference. Fig. S10: photostability of AIE probes in PBS under the continuous natural light irradiation for 30 days. Fig. S11: body weight of cynomolgus monkeys during the experiment period. Fig. S12: body temperature of cynomolgus monkeys during the experiment period. Fig. S13: fluorescence signals of feces collected at different time points. Fig. S14: ex vivo NIR-II fluorescence imaging of the liver, spleen, and lymph nodes of AIE probe-treated cynomolgus monkey at the end of a 35-day postinjection. Fig. S15: ex vivo NIR-II fluorescence imaging of the muscle, stomach, brain, heart, intestine, kidney, and lung of AIE probe-treated cynomolgus monkey at the end of a 35-day postinjection. Fig. S16: dynamic NIR-II fluorescence imaging of blood flow at with 5 frames per second. Fig. S17: dynamic NIR-II fluorescence imaging of blood flow at with 5 frames per second. Table S1: summary of reported NIR-II AIE probes for in vivo fluorescence imaging.
Supplementary 2. Video 1: NIR-II vascular perfusion imaging.
Supplementary 3. Video 2: NIR-II vascular imaging at 1.5-centimeter depth.
- V. Ntziachristos, C. H. Tung, C. Bremer, and R. Weissleder, “Fluorescence molecular tomography resolves protease activity in vivo,” Nature Medicine, vol. 8, no. 7, pp. 757–761, 2002.
- J. C. Vaughan and X. Zhuang, “New fluorescent probes for super-resolution imaging,” Nature Biotechnology, vol. 29, no. 10, pp. 880-881, 2011.
- R. Weissleder and V. Ntziachristos, “Shedding light onto live molecular targets,” Nature Medicine, vol. 9, no. 1, pp. 123–128, 2003.
- W. Z. Yuan, P. Lu, S. Chen et al., “Changing the behavior of chromophores from aggregation-caused quenching to aggregation-induced emission: development of highly efficient light emitters in the solid state,” Advanced Materials, vol. 22, no. 19, pp. 2159–2163, 2010.
- J. Luo, Z. Xie, J. W. Lam et al., “Aggregation-induced emission of 1-methyl-1,2,3,4,5-pentaphenylsilole,” Chemical Communications, no. 18, pp. 1740-1741, 2001.
- Y. Hong, J. W. Lam, and B. Z. Tang, “Aggregation-induced emission: phenomenon, mechanism and applications,” Chemical Communications, no. 29, pp. 4332–4353, 2009.
- W. Qin, D. Ding, J. Liu et al., “Biocompatible nanoparticles with aggregation-induced emission characteristics as far-red/near-infrared fluorescent bioprobes for in vitro and in vivo imaging applications,” Advanced Functional Materials, vol. 22, no. 4, pp. 771–779, 2012.
- Z. Li and A. Qin, “Diverge from the norm,” National Science Review, vol. 1, no. 1, pp. 22–24, 2014.
- J. Qian and B. Z. Tang, “AIE luminogens for bioimaging and theranostics: from organelles to animals,” Chem, vol. 3, no. 1, pp. 56–91, 2017.
- D. Ding, K. Li, B. Liu, and B. Z. Tang, “Bioprobes based on AIE fluorogens,” Accounts of Chemical Research, vol. 46, no. 11, pp. 2441–2453, 2013.
- J. Mei, N. L. Leung, R. T. Kwok, J. W. Lam, and B. Z. Tang, “Aggregation-induced emission: together we shine, united we soar!,” Chemical Reviews, vol. 115, no. 21, pp. 11718–11940, 2015.
- X. Lim, “The nanolight revolution is coming,” Nature, vol. 531, no. 7592, pp. 26–28, 2016.
- Z. Sheng, B. Guo, D. Hu et al., “Bright aggregation-induced-emission dots for targeted synergetic NIR-II fluorescence and NIR-I photoacoustic imaging of orthotopic brain tumors,” Advanced Materials, vol. 30, no. 29, article 1800766, 2018.
- Y. Li, Z. Cai, S. Liu et al., “Design of AIEgens for near-infrared IIb imaging through structural modulation at molecular and morphological levels,” Nature Communications, vol. 11, no. 1, article 1255, 2020.
- J. Qi, C. Sun, A. Zebibula et al., “Real-time and high-resolution bioimaging with bright aggregation-induced emission dots in short-wave infrared region,” Advanced Materials, vol. 30, no. 12, article 706856, 2018.
- S. Gao, G. Wei, S. Zhang et al., “Albumin tailoring fluorescence and photothermal conversion effect of near-infrared-II fluorophore with aggregation-induced emission characteristics,” Nature Communications, vol. 10, no. 1, pp. 2206–2215, 2019.
- W. Xu, D. Wang, and B. Z. Tang, “NIR-II AIEgens: A Win-Win Integration towards Bioapplications,” Angewandte Chemie International Edition, 2020.
- J. Qi, N. Alifu, A. Zebibula et al., “Highly stable and bright AIE dots for NIR-II deciphering of living rats,” Nano Today, vol. 34, article 100893, 2020.
- G. Hong, A. L. Antaris, and H. Dai, “Near-infrared fluorophores for biomedical imaging,” Nature Biomedical Engineering, vol. 1, no. 1, pp. 1–22, 2017.
- C. Li and Q. Wang, “Challenges and opportunities for intravital near-infrared fluorescence imaging technology in the second transparency window,” ACS Nano, vol. 12, no. 10, pp. 9654–9659, 2018.
- G. Hong, S. Diao, J. Chang et al., “Through-skull fluorescence imaging of the brain in a new near-infrared window,” Nature Photonics, vol. 8, no. 9, pp. 723–730, 2014.
- G. Hong, J. C. Lee, J. T. Robinson et al., “Multifunctional in vivo vascular imaging using near-infrared II fluorescence,” Nature Medicine, vol. 18, no. 12, pp. 1841–1846, 2012.
- K. Welsher, Z. Liu, S. P. Sherlock et al., “A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice,” Nature Nanotechnology, vol. 4, no. 11, pp. 773–780, 2009.
- A. L. Antaris, H. Chen, K. Cheng et al., “A small-molecule dye for NIR-II imaging,” Nature Materials, vol. 15, no. 2, pp. 235–242, 2016.
- J. A. Carr, D. Franke, J. R. Caram et al., “Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green,” Proceedings of the National Academy of Sciences, vol. 115, no. 17, pp. 4465–4470, 2018.
- Z. Hu, C. Fang, B. Li et al., “First-in-human liver-tumour surgery guided by multispectral fluorescence imaging in the visible and near-infrared-I/II windows,” Nature Biomedical Engineering, vol. 4, no. 3, pp. 259–271, 2020.
- J. Bluemel, S. Korte, E. Schenck, and G. Weinbauer, The Nonhuman Primate in Nonclinical Drug Development and Safety Assessment, Academic Press, 2015.
- Y. Lu, Y. J. Xu, G. B. Zhang et al., “Iron oxide nanoclusters for T1 magnetic resonance imaging of non-human primates,” Nature Biomedical Engineering, vol. 1, no. 8, pp. 637–643, 2017.
- L. Ye, K. T. Yong, L. Liu et al., “A pilot study in non-human primates shows no adverse response to intravenous injection of quantum dots,” Nature Nanotechnology, vol. 7, no. 7, pp. 453–458, 2012.
- J. Liu, F. Erogbogbo, K. T. Yong et al., “Assessing clinical prospects of silicon quantum dots: studies in mice and monkeys,” ACS Nano, vol. 7, no. 8, pp. 7303–7310, 2013.
- J. Xu, M. Yu, C. Peng et al., “Dose dependencies and biocompatibility of renal clearable gold nanoparticles: from mice to non-human primates,” Angewandte Chemie International Edition, vol. 57, no. 1, pp. 266–271, 2018.
- L. Moore, J. Yang, T. T. H. Lan et al., “Biocompatibility assessment of detonation nanodiamond in non-human primates and rats using histological, hematologic, and urine analysis,” ACS Nano, vol. 10, no. 8, pp. 7385–7400, 2016.
- M. Yu, J. Xu, and J. Zheng, “Renal clearable luminescent gold nanoparticles: from the bench to the clinic,” Angewandte Chemie International Edition, vol. 58, no. 13, pp. 4112–4128, 2019.
- H. Hong, F. Chen, and W. Cai, “Pharmacokinetic issues of imaging with nanoparticles: focusing on carbon nanotubes and quantum dots,” Molecular Imaging and Biology, vol. 15, no. 5, pp. 507–520, 2013.
- H. F. Krug, “Nanosafety research—are we on the right track?” Angewandte Chemie International Edition, vol. 53, no. 46, pp. 12304–12319, 2014.
- Y. Hong, J. W. Lam, and B. Z. Tang, “Aggregation-induced emission,” Chemical Society Reviews, vol. 40, no. 11, pp. 5361–5388, 2011.
- M. Zhang, J. Yue, R. Cui et al., “Bright quantum dots emitting at ∼1,600 nm in the NIR-IIb window for deep tissue fluorescence imaging,” Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 26, pp. 6590–6595, 2018.
- A. M. Smith, M. C. Mancini, and S. Nie, “Second window for in vivo imaging,” Nature Nanotechnology, vol. 4, no. 11, pp. 710-711, 2009.
- C. Li, “A targeted approach to cancer imaging and therapy,” Nature Materials, vol. 13, no. 2, pp. 110–115, 2014.
- S. Kim, Y. T. Lim, E. G. Soltesz et al., “Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping,” Nature Biotechnology, vol. 22, no. 1, pp. 93–97, 2004.
- D. M. McDonald and P. L. Choyke, “Imaging of angiogenesis: from microscope to clinic,” Nature Medicine, vol. 9, no. 6, pp. 713–725, 2003.
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