Responsive Trimodal Probes for In Vivo Imaging of Liver Inflammation by Coassembly and GSH-Driven Disassembly
Response of GdNPs-Gal toward GSH. (a) HPLC analysis of 1-Ga, 1-Gal, and GdNPs-Gal before and after incubation with GSH. (b) DLS analysis of GdNPs-Gal (200 μM) following incubation with GSH (10 mM) for 0–50 min. (c) Plots of 1/ versus Gd concentration determine the relaxivities of GdNPs-Gal before (black) and after (red) incubation with GSH (10 mM, 60 min). Inset: -weighted MR images of the following: I: PBS; II: GdNPs-Gal (200 μM); III: ; IV: Dotarem (200 μM). (d) values, (e) fluorescence spectra, and (f) 19F NMR spectra of GdNPs-Gal (200 μM) upon incubation with GSH (10 mM) for 0–60 min. (g) values, (h) fluorescence spectra, and (i) 19F NMR spectra of GdNPs-Gal (200 μM) upon incubation with varying concentrations of GSH for 60 min. The fluorescence spectra of GdNPs-Gal were measured after 1 to 10 dilutions with PBS buffer. Data denote ().