Research / 2020 / Article / Fig 5

Research Article

Responsive Trimodal Probes for In Vivo Imaging of Liver Inflammation by Coassembly and GSH-Driven Disassembly

Figure 5

Detection of GSH in living mice with liver inflammation using GdNPs-Gal. (a) Longitudinal, coronal, and axial -weighted MR images of livers in healthy mice receiving i.v. injection of 0.1 mmol kg−1 of GdNPs-Gal (up) or GdNPs-Gal-Ctrl (down). Images were acquired before (pre), 1, 2, 4, 6, and 8 h after injection. The MR contrast in livers is shown in pseudo rainbow color for comparison; white arrows in axial images indicate the Dotarem (1 mM) solution as the internal standard. (b) Average % SE in livers of healthy mice after i.v. injection of GdNPs-Gal or GdNPs-Gal-Ctrl. (c) Schematic illustration of MRI of hepatic GSH in LPS-induced inflammatory mice. (d) Measurement of TNF-α (grey) and IL-1β (red) in mouse serums before and 6 h after i.p. injection of LPS (20 mg kg−1). (e) Quantification of hepatic GSH level before and 6 h after i.p. injection of LPS. (f) Coronal and axial -weighted MR images and (g) averaged % SE of livers in healthy mice (LPS (-)) or inflammatory mice (LPS (+)) at 4 h post i.v. injection of GdNPs-Gal (0.1 mmol kg−1). The MR contrast in livers is shown in pseudo rainbow color for comparison; white arrows in axial images indicate the Dotarem (1 mM) solution as the internal standard. (h) Fluorescence images of liver tissue slices and (i) 19F NMR spectra of liver tissue homogenates dissected from healthy and inflammatory mice at 4 h post i.v. injection of GdNPs-Gal (0.1 mmol kg−1). Scale bars: 100 μM. Data denote (, ).
(a)
(b)
(c)
(d)
(e)
(f)
(g)
(h)
(i)

Please note that the Table of Contents page is currently not displaying content. We are working to fix this issue as soon as possible and apologize for the inconvenience.