Photothermally Responsive Conjugated Polymeric Singlet Oxygen Carrier for Phase Change-Controlled and Sustainable Phototherapy for Hypoxic Tumor
In vitro evaluation of CP1-NCs under 21% or 5% oxygen concentration. (a) MTT assay of CP1-NCs with and without irradiation. (b) Confocal image of HeLa cells cultured with CP1-NCs with or without 5 min light irradiation (690 nm, 0.5 W cm-2) using DCFH-DA staining (,, scale bar: 100 μm). (c) Calcein AM and PI-stained HeLa tumor cells treated with CP1-NCs with or without 5 min light irradiation (690 nm, 0.5 W cm-2, scale bar: 100 μm). (d) Flow cytometry quantification of apoptosis of HeLa cells incubated with CP1-NCs under 690 nm light irradiation (0.5 W cm-2).