Research / 2020 / Article / Fig 5

Research Article

Semi-Quantitatively Designing Two-Photon High-Performance Fluorescent Probes for Glutathione S-Transferases

Figure 5

(a–c) Single-photon confocal fluorescence imaging of HepG2 cells (a) incubated with 20 μM NI3, (b) pretreated with 100 μM EA and then incubated with 20 μM NI3, and (c) pretreated with 50 μM NEM and then incubated with 20 μM NI3 with a 100x objective. λex = 458 nm. λem = 500–600 nm. Scale bar = 20 μm. (d) Flow cytometric analysis of the HepG2 cells. λex = 488 nm. λem = 500–600 nm. (e) Assay of the HepG2 cell lysate using 20 μM NI3 with 1 mM GSH added in. “Control” means assay without cell lysate. λex = 445 nm. λem = 560 nm. (f–i) Fluorescence images of various cell lines incubated with 20 μM NI3 for 30 min with a 40x objective. λex = 458 nm. λem = 500–600 nm. Scale bar = 20 μm. Representative images from repeated experiments are shown.

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