Semi-Quantitatively Designing Two-Photon High-Performance Fluorescent Probes for Glutathione S-Transferases
Experimental and theoretical studies on the fluorescence quenching mechanism. (a) Pseudocolor femtosecond transient absorption (TA) spectral plot of NI9 in DMSO. (b) Kinetic traces at different wavelengths following the 370 nm laser pulse excitation and the respective fit with three exponential functions. (c) TD-DFT calculations on the electronic transitions of NI9 and NI in DMSO at the B3LYP/aug-cc-pVDZ level.
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