Research / 2020 / Article / Fig 3

Research Article

A Versatile Surface Bioengineering Strategy Based on Mussel-Inspired and Bioclickable Peptide Mimic

Figure 3

(a) Structural formula of the DBCO-modified antibacterial peptide (DBCO-ABP). (b) ESI mass spectrum of the DBCO-ABP. (c) Bioorthogonal conjugation of DBCO-ABP on DOPA4-azide-coated PVC substrates. (d) GATR-FTIR spectra of the DOPA4-azide-coated and ABP-modified surfaces. (e) XPS analysis of the PVC substrates after each step of surface treatments. (f) Agar plates observed after 24 h incubation of E. coli and S. aureus on the bare, DOPA4-azide-coated, and ABP-modified PVC substrates, respectively (plate sizes: 10 cm). (g) Photographs of the bacterial media after 12 h incubation with bare, DOPA4-azide-coated, and ABP-modified PVC substrates, respectively. (h) Quantitative analysis of bacterial killing efficiency by measuring the optical density at 600 nm based on the turbidity of the bacterial suspension. Statistically significant differences are indicated by .