Synergistically Enhanced Mucoadhesive and Penetrable Polypeptide Nanogel for Efficient Drug Delivery to Orthotopic Bladder Cancer
In vitro cell uptake and cytotoxicity. (a) Representative CLSM images of human BC 5637 cells incubated with free HCPT, NG/HCPT, or R9NG/HCPT for 2 or 6 h. The blue color was HCPT fluorescence in cells (left). Differential interference contrast (DIC) was used to obtain the bright-field images (middle), and the merged images were shown on the right. The scale bar represents 50 μm. (b) Intracellular HCPT release profiles in human BC 5637 cells after co-incubation with free HCPT, NG/HCPT, or R9NG/HCPT. Data are presented as (;,, and ). (c) The viability of human BC 5637 cells incubated with free HCPT, NG/HCPT, or R9NG/HCPT at different concentrations for 24 h. Data are presented as (;,). (d) Apoptotic cell populations were calculated by FCM analysis after co-incubating human BC 5637 cells with PBS as a control, free HCPT, NG/HCPT, or R9NG/HCPT for 24 h. The lower-left (Q3), lower-right (Q4), upper-right (Q1), and upper-left (Q2) quadrants in each panel indicated the populations of healthy, early and late apoptotic, and necrotic cells, respectively.