Research / 2020 / Article / Fig 2

Research Article

Spheroids of Endothelial Cells and Vascular Smooth Muscle Cells Promote Cell Migration in Hyaluronic Acid and Fibrinogen Composite Hydrogels

Figure 2

(a) Fluorescent images of (1, 4, 7) EC, (2, 5, 8) SMC, and (3, 6, 9) EC-SMC spheroids after being stained by (1-3) FDA and (4-6) PI, respectively. (7-9) Corresponding merged images. These cell spheroids were just transferred into normal culture plate. Green and red represent live and dead cells, respectively. Scale bar: 200 μm. (b) CLSM images of (1) EC, (2) SMC, and (3) EC-SMC spheroids after being cultured for 3 days on TCPS, respectively. The cytoskeleton and cell nuclei were stained with rhodamine-labeled phalloidin (red) and DAPI (green), respectively. (c) Cytoviability and proliferation assay of the cell spheroids. (1) MTT assay and (2) cell number after the cell spheroids were cultured on TCPS for different times. (3) Viability of EC, SMC, and EC-SMC spheroids after they were cultured on the surface of HA-MA/Fg(1/1) composite hydrogel for different times.