Endothelium-Mimicking Multifunctional Coating Modified Cardiovascular Stents via a Stepwise Metal-Catechol-(Amine) Surface Engineering Strategy
In vitro blood compatibility tests. (a) Relative quantify data of fibrinogen adsorption and γ chain exposure on 316L SS, Hep, NO, and Hep@NO samples. (b) The related cGMP expression in platelets after incubation of platelet-rich plasma (PRP) with samples for 30 min at 37°C, tested by ELISA kit. NO donor solution (10 μM GSNO and 10 μM GSH, PBS) was added to PRP. (c) Morphology of platelets on samples determined by scanning electron microscope (SEM). (d) Representative images of P-selectin staining, where activated platelets were marked by green fluorescence. (e) The numbers of the adherent and activated platelets on samples. Data exhibited as the ( or ) and analyzed using a one-way ANOVA; ,, and compared to 316L SS; # and ### compared to Hep; & and && compared to NO.