Biofilm Microenvironment-Responsive Nanotheranostics for Dual-Mode Imaging and Hypoxia-Relief-Enhanced Photodynamic Therapy of Bacterial Infections
Characterization and drug release of MBP-Ce6 NSs. (a) The fluorescence spectra of Ce6 aqueous solutions (20 μg/mL) after being mixed with MnO2 NSs (0, 6, 12, 18, 24, 30, 36, and 48 μg/mL). (b) The UV-Vis-NIR spectra of Ce6, MnO2 NSs, MBP NSs, and MBP-Ce6 NSs. (c) The loading efficiency and encapsulation efficiency of Ce6 at different feeding ratios of Ce6 and MnO2 NSs. (d) The TEM images of MBP-Ce6 NSs after being stored in PBS at different conditions. The scale bar is 100 nm for all TEM images. (e) The time-dependent degradation of MBP NSs (40 μg/mL) in PBS at different pH values. (f) Released Ce6 from MBP-Ce6 NSs over time at different conditions. (g) The time-dependent concentration change of dissolved O2 in H2O2 solutions (50 μM) after being mixed with MBP-Ce6 NSs at different concentrations (0, 5, 10, and 20 μg/mL). Error bars indicate s.d. ().