Research / 2022 / Article / Fig 2

Research Article

Site-Specific Photochemical Reaction for Improved C=C Location Analysis of Unsaturated Lipids by Ultraviolet Photodissociation

Figure 2

(a) The structure of PC 16 : 0/18 : 1 (9Z) and the C=C diagnostic fragments generated from Atp, Bzp, or 4-CF3-Bzp as the PB reagents; (b) 210 nm UVPD MS/MS spectrum of PC 16 : 0/18 : 1 (9Z) after 10 pulses; (c) CID MS/MS spectrum of PB product ions of PC 16 : 0/18 : 1 (9Z) derivatized by 4-CF3-Bzp with collision energy applied at 25 eV; UVPD MS/MS spectra of PB products of PC 16 : 0/18 : 1 (9Z) with (d, e) Bzp, (f, g) 4-CF3 Bzp, or (h, i) Atp at 210 nm and 260 nm after 2 pulses. The laser energies were 0.5–1.0 mJ per pulse. C=C diagnostic ions denoted as 9FA and 9FO were labeled as olive and orange, respectively. Pulse-dependent efficiencies for photodissociation of Bzp or 4-CF3-Bzp modified PC 16 : 0/18 : 1 (9Z) lipid are given in Figure S3.