Research / 2022 / Article / Fig 4

Research Article

Site-Specific Photochemical Reaction for Improved C=C Location Analysis of Unsaturated Lipids by Ultraviolet Photodissociation

Figure 4

UVPD MS/MS spectra of the PB products of (a, b) PE 16 : 0/18 : 1 (9Z), (c, d) PG 16 : 0/18 : 1 (9Z), (e, f) PS 16 : 0/18 : 1 (9Z), or (g, h) PA 16 : 0/18 : 1 (9Z) with 4-CF3-Bzp at 210 nm and 225 nm after 2 pulses. Protonated diagnostic ion pair 9FA/O of C=C bond was labelled as red, and the corresponding headgroup-liberated C=C diagnostic pair 9fA/O was colored as blue. NH4+-cationized diagnostic ions were also observed for (c, d) PG and (g, h) PA and labelled as olive. The headgroups of PE, PG, PS, and PA were denoted as PhE, PhG, PhS, and Ph, respectively. Wavelength-dependent photodissociation profiles and pulse-dependent photodissociation efficiency of the PB products of PE 16 : 0/18 : 1 (9Z) with 4-CF3-Bzp can be found in Figure S9.