Localized Myocardial Anti-Inflammatory Effects of Temperature-Sensitive Budesonide Nanoparticles during Radiofrequency Catheter Ablation
Biocompatibility and effectiveness verification in vitro. (a) Fluorescence images of stained cardiac fibroblasts in the different group. (Cytoskeleton: phalloidin, red fluorescence; cell nucleus: DAPI, blue fluorescence; live cells: calcein-AM, green fluorescence; dead cells: PI, red fluorescence, ). (b) The intracellular uptake of P/B-COS-Cy5 NPs by RAW264.7 cells after incubation with the different times and detected by confocal laser scanning microscope (cell nucleus: Hoechst, blue fluorescence; P/B-COS-Cy5 NPs: ed fluorescence, ). (c) Fluorescence quantitative analysis of the intracellular uptake of NPs in (b). (d) The cytotoxicity evaluation of NPs using CCK-8 assay in cardiac fibroblasts with different concentration (,). (e) TNF-α and (f) IL-6 concentration in culture supernatant. Evaluation of anti-inflammatory effect of NPs in RAW264.7 cell (,, indicates , indicates ).